Prolactin (PRL) is a neuroendocrine hormone that is closely related to growth hormones.
The endocrine effects of PRL on human breast tissues include the regulation of growth and differentiation of ductal epithelium, proliferation and differentiation of lobular units, and initiation and maintenance of lactation..
Both the PRLr and growth hormone receptors are single-chain transmembrane proteins composed of extracellular, transmembrane, and intracellular domains.
Prolactin modulates immune system function by stimulating the proliferation and survival of cells through its receptor, present on T and B lymphocytes and macrophages.
The function of PRL is mediated by a variety of signaling cascades as well as by the wide variety of PRLr isoforms observed in nature.
A long form and several other isoforms are expressed in human tissues.
The nucleotide sequence of the novel intermediate isoform of PRLr is identical to the long isoform, except for a 573-base pair deletion occurring at a consensus splice site, resulting in a frameshift and truncated intracytoplasmic domain.
The long isoform of PRLr is N-glycosylated.
As the extracellular domain of the intermediate isoform is identical to that of the long isoform, glycosylation patterns are assumed to be similar.
Using immunohistochemical analysis, PRLr expression has been observed in 95% of both normal and malignant breast epithelium; no correlation between PRL expression and ER-PR status has been established.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunopreciptation: 10ug/IP reactionOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes the unique sequence of the human prolactin receptor (PRLr) intermediate isoform. Reactivity is confirmed with T47D breast carcinoma cell line.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the C-terminal sequence of human prolactin receptor intermediate isoform.
