The P2 purinoreceptor family consists of two distinct classes: the P2X ligand-gated ion channels, and the P2Y G-protein coupled receptors (GPCR).
Eleven subtypes of P2Y receptors have been identified; five are recognized in mammalian species.
P2Y receptors are activated by extracellular adenine (ATP) and uridine (UTP) nucleotides.
Binding of adenine or uridine to the P2Y receptor triggers a conformational change and the activation of the associated G protein, which inducesphospholipase C (PLC) stimulation, the release of intracellular calcium ions, and the opening of plasma membrane chloride channels.
The P2Y1 receptor is composed of an intracellular C-terminus, seven transmembrane domains with three intracellular and three extracellular loops, and an extracellular N-terminus with four conserved cysteine residues for disulfide bond formation.
Disulfide bridges between the first and second extracellular loops and between the N-terminus and the third extracellular loop are necessary for both structure and stability of the P2Y1 receptor.
The extracellular loops are essential for ligand recognition and receptor activation.
ATP docking sites have been identified in the transmembrane cleft and at meta binding sites within the extracellular loops.
P2Y1 receptor protein expression has been observed in a variety of mammalian tissues and cells.
In humans, P2Y1 is present in the CNS (neurons of the hippocampus, midbrain, cerebellar cortex, and cerebral cortex), heart muscle, skeletal muscle, and some types of smooth muscle, myeloid and leukemia cells, and kidney cells.
In murine species, the P2Y2 receptor is expressed in rat heart and brain.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/mlELISA: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes the mouse and rat P2Y1 receptor proteins. Reactivity is confirmed with mouse brain homogenates. On Western blots, a strong clear band ~63kD is exhibited. This molecular weight corresponds to a published report of the P2Y1 receptor; however, other publications have described this protein at 40 and 42kD.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the intracellular C-terminal region of the human P2Y1 receptor protein. Species sequence homology: human, bovine
