The NFkB family of induced-activated transcription factors are dimers comprised of members of the NFkB /rel protein family.
Activation of NFkB is induced by a remarkably large number of stimuli including viruses, TNF, IL-1, PMA, LPS, UV light, and others.
In most cell types, NFkB is present in the cytoplasm as a 50 kD (p50) subunit and a 65 kD (p65, rel A) subunit.
Inunstimulated cells, nuclear localization signals present on p65 are masked by members of the IkB family of inhibitory proteins.
Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosomedependant pathway, allowing nuclear translocation of the active dimeric NFkB transcription factor.
In the nucleus, NFkB binds to consensus sequences where, along with other co-factors, it participates in activating or enhancing the expression of specific genes.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:ELISA (Immunogen): 0.1-1ug/mlWestern Blot (Human and monkey): 1-3ug/mlImmunohistochemistry (FFPE) (human): 0.5-2ug/ml.
Heat-induced epitope retrieval (Citrate buffer, pH 6.0) is requiredImmunoprecipitation: 2-5ug/IPOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes the DNA binding subunit of human NFkB (also referred to as Rel A) at ~65kD.. Reactivity with other endogenous proteins has not been detected. Species Crossreactivity: monkey. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mk
Immunogen：A recombinant protein derived from the carboxyl terminal portion of the human NFkB protein.
