(IRAKs) are important mediators in the signal transduction of the Toll/IL-1 receptor (TIR) family.
Members of the TIL family play a role in innate immune responses by coordinating host defense mechanisms.
IRAK4 is the most recently discovered IRAK protein.
IRAK-4 has been shown to interact with IRAK-1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK-4 has been shown to activate NFkB as well as mitogen-activated protein (MAP) kinase pathways.
Studies demonstrate that mice lacking IRAK4 are severely impaired in their IL-1, Toll-like receptor (TLR) signaling and response to viral and bacterial challenges; this indicates that IRAK4 has an important role in innate immunity.
Humans lacking IRAK 4 exhibit pyogenic bacterial infections, while their blood and fibroblast cells fail to activate NF-kB.
IRAK-4 is believed to be required for the LPS-induced activation of antigen-presenting cells.
Applications:Suitable for use in Western Blot and Immunofluorescence.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1-3ug/mlImmunofluorescence: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes the human IRAK4 protein. On Western Blots, it identifies a single band at ~52kD. Reactivity has been confirmed with human HeLa cell lysates. Reactivity with mouse and rat is expected based on sequence homology.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the N-terminal region of the human IRAK4 protein. Species Sequence Homology: mouse and rat, 100%
