Eph receptors belong to a family of receptor tyrosine kinases.
Both ephrins, the ligands for Eph receptors, and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos.
The interaction of ephrins with the appropriate Eph receptors has been implicated in a wide spectrum of functions in vertebrate morphogenesis, including the accurate migration of embryonic cells, formation of boundaries between structures (i.e.
rhombomeres and somites), and the control of cytoskeletal changes which dictate cellular shape and adhesion during development.
EphA3 receptor, also known as Cek4, Mek4, Hek, Tyro4 and Hek4, is thought to repel receptor positive myoblasts and axons away from the limb bud and towards the body wall.
EphA3 receptor is involved in targeting of the retinotectal projection and the organization of the retinotectal pathway.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by epitope affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Reacts with the ~110kD (calculated molecular weight) human EphA3 receptor.Reactivity is confirmed with human HEK 293 cells carrying an expression vector encoding human EphA3 receptor. Based on sequence homology, this antibody is expected to react with mouse and rat EphA3 receptor. No cross-reactivity was observed with human EphA2, EphA4, EphB2, EphB4 or EphB5 receptors by Western blotting.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the C-terminal region of the human EphA3 receptor protein.
