84-3653-55　Claudin 10 (CLDN10) (FITC) 100ul　C5838-60-FITC

特徴

• Tight junctions are intercellular junctions that act as both the barrier (or gate), and the fence.
• The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces.
• The fence function maintains cell polarity, thus, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane.
• Tight junctions are comprised of the membrane proteins occludin, claudins, and JAMs.
• Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton mediated by ZO-1.
• Claudin 10 is concentrated at the tight junctions of epithelial cells of AQP1-positive tubules in the kidney cortex (proximal tubules).
• Claudin 10 is also detected at the tight junctions of epithelial cells of THP-positive tubules in the kidney medulla (thick ascending limb of Henle).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Immunohistochemistry: Frozen and paraffin sectionsOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
• Note: Applications are based on unconjugated antibody.