84-3653-49　Cks1, CT (Cdc kinase subunit 1) (FITC) 100ul　C5817-FITC

特徴

• Cks (Cdc kinase subunit ) proteins are members of the cyclin-dependent kinase (cdk) family that act as adaptors to allow the cdks to interact more efficiently with their molecular targets.
• Cks1 is involved in the regulation of cell cycle mitosis in normal and cancer cells.
• Cks1 promotes mitosis by modulating the transcriptional activation of the APC/C protein-ubiquitin ligase activator Cdc20.
• Cks1 has also been established as an essential cofactor in the ubiquitylation of Cdk inhibitor p27 by Skp2 ubiquitin ligase.
• Mammalian Cks1 binds to Skp2 and promotes the association of Skp2 with phosphorylated p27 (Thr187), which further induces ubiquitylation of p27.
• High expression of Cks1 may be involved in the pathogenesis of human non-small cell lung carcinoma.
• Applications:Suitable for use in FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Immunohistochemistry: Frozen and paraffin sectionsOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.