Cdc14, also known as duel specificity phosphatase, is a protein phosphatase originally discovered in yeast that plays an important role in cell cycle regulation, including exit from mitosis.
Throughout G1, S and G2 phases, Cdc14 is located in the nucleolus; at the beginning of anaphase, the protein is redistributed throughout the entire nucleus.
In late anaphase, Cdc14dephosphorylates Cdh1 which then activates APC (Anaphase Promoting Complex).
Two human orthologs of yeast Cdc14 have been identified; these are Cdc14A and Cdc14B, and they share about 80% amino acid homology in the N-termini and phosphatase domains.
Cdc14 has also been shown to dephosphorylate p53, suggesting that it may play a role in regulation of p53 activity.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunofluorescence: 1-5ug/mlWestern Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by epitope affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Reacts with the ~80kD human Cdc14A protein. Reactivity is confirmed with human HEK293 cell lysates.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the C-terminal region of the human Cdc14A protein.
