Ubiquitin carboxyl-terminal hydrolase L1 (UCH–L1), also known as PGP 9.5, is a neuronal and neuroendocrine-specific protein that plays a role in proteosomal protein degradation, which is critical for neuronal health.
UCH-L1 is also involved in p27kip1 degradation through its interaction and nuclear translocation with JAB1.
UCH-L1 expression is specifically associated with non small-cell lung cancer development and it is found in serum of patients with lung cancer, indicating that it may be important in lung cancer screening and diagnosis UCH-L1 may also be a useful marker for invasive colorectal cancer.
Mutations in the alpha-synuclein and Uch-L1 genes cause autosomal dominant Parkinson’s disease (PD).
Derangements in the ubiquitin proteasomal pathway may play important roles in the demise of dopamine neurons in PD.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.1-1ug/mlImmunohistochemistry (Paraffin): 1-2ug/ml; For staining with formalin-fixed paraffin-embedded tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining
Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes the ~26kD human UCH-L1 protein. A faint band of unknown origin may also be observed at ~50kD. Reactivity has been confirmed with mouse and rat brain homogenates, NGP neuroblastoma lysates, A549 lung carcinoma lysates, and NCI-H69 lung carcinoma lysates. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from the C-terminal region of human UCH-L1. Species Sequence Homology: mouse, rat, equine; 100%
