Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Recognizes soluble and membrane-bound forms of human Neuropilin-1. On Western Blots, it identifies two bands, one at ~130–135kD representing the membrane-bound form and another at ~90kD for the soluble form. Reacts with human PC-3 prostate adenocarcinoma cell lysates. Species Sequence Homology: Chicken and frog Npn-1 (Mouse and rat crossreactivity is also possible).
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide derived from the N-terminal region of the human Neuropilin-1 protein.