84-3653-21　Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301) (FITC) 100ul　J8009-FITC

特徴

• The junctional adhesion molecule-1 (JAM-1) is an integral membrane protein that colocalizes with tight junction molecules occludin, ZO-1, and cingulin.1 JAM-1 has been characterized as a member of the immunoglobulin (Ig) superfamily and is composed of an extracellular domain, a single-pass transmembrane region, and a short cytoplasmic tail.2 JAM-1 is localized at tight junctions in endothelial and epithelial cells and its sub-cellular distribution is modulated by inflammatory cytokines.2 JAM-1 is also expressed on circulating leukocytes and platelets as well as in lymphoid.2 Most claudins as well as JAM-1 end with the amino acid Val at their C-terminal ends, suggesting that these COOH termini directly bind to PDZ domains.3 JAM-1 appears to be a single copy gene, which is constitutively expressed on various tissues and cells, and may be involved in cell-to-cell adhesion through homophilic interaction.4 JAM-1 was detected in human intestine cell lines T84, CaCO2 and HT29, in the lung cell line A 549, and the liver cell line HepG2.5
• Applications:Suitable for use in Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blotting: 1–3ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.