Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.
Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.
Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.
This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
The anti-epitope tag antibody is usually functional in a variety of antibody- dependent experimental procedures.
Expression vectors producing epitope tagfusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-1.0ug/ml Immunoprecipitation: 5ug Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Also Available:H1200-01: HA epitope, fusion tag Pab Rb xRbH1200-02: HA epitope, fusion tag, Control PeptideH1200-03: HA epitope, fusion tag Mab Mo xH1400: HA epitope, fusion tag Pab Rb xH1847-51: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) Pab Ch xH1847-51B: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (FITC) Mab Mo xH1847-52: Hemagglutinin Tag (CYPYDVPDYASL) (HA Tag) Pab Rb xH1847-52A: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) Pab Ch xH1847-52B: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) Pab Gt xH1847-52C: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) Pab Rb xH1847-52D: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (FITC) Pab Gt xH1847-52E: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (HRP) Pab Gt xH1847-52F: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (HRP) Pab Rb xH1847-52G: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (Agarose) Pab Gt xH1847-52X: Hemagglutinin Tag (CYPYDVPDYASL) (HA Tag) (BSA & Azide Free) Pab Rb xH1847-53: Hemagglutinin Tag (YPYDVPDYA) (HA tag) Pab Rb xH1847-53A: Hemagglutinin Tag (YPYDVPDYA) (HA Tag) Mab Mo xH1847-53X: Hemagglutinin Tag (YPYDVPDYA) (HA tag) Mab Mo xH1847-54: Hemagglutinin Tag (CYPYDVPDY) (HA tag) Mab Rb xH1847-55: Hemagglutinin Tag (YPYDVPDYA) (HA tag) Mab Mo xI7650-17A: Hemagglutinin (HA) EPITOPE TAG (IRDye™800) Pab Rb xI7650-17B: Hemagglutinin (HA) Epitope Tag (Biotin) Pab Rb xI7650-17C: Hemagglutinin (HA) EPITOPE TAG (IRDye™800CW) Pab Rb x
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes recombinant proteins containing the HA epitope tag: YPYDVPDYA. Recognizes the HA-tag fused to either the N- or C-termini of targeted proteins. Species Crossreactivity: A broad species crossreactivity is expected based on conservation of sequence homology.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Immunogen：Synthetic peptide containing the sequence YPYDVPDYA derived from the influenza virus hemaglutinin (HA) protein (KLH).
