84-3653-07　［取扱停止］Ephrin B1 (Ephrin-B1, ELK-L, EFL-3, Cek5-L, LERK-2) (FITC) 100ul　E3371-FITC

特徴

• Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family.
• Both ephrins and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos.1 The interaction of ephrins with the appropriate Eph receptor(s) has been implicated in a wide spectrum of functions in vertebrate morphogenesis, including the accurate migration of embryonic cells,2 formation of boundaries between structures (i.e.
• rhombomeres and somites), and the control of cytoskeletal changes which dictate cellular shape and adhesion during development.
• Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors; Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors.
• Ephrin-B1, also known as ELK-L, EFL-3, Cek5-L, LERK-2, and STRA-13 is a Class B protein.
• It has been shown to bind the EphB1, EphB2, EphB3, EphB4, and EphA3 receptors.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1-5ug/ml Immunoprecipitation: 5-10ug/IP reaction Immunohistochemistry (FFPE): 5-10ug/ml.
• Pepsin digestion is required for optimal staining
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.