84-3652-97　NFkB, p50 (NF-kB, Nuclear Factor kappa B p50 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B cells 1, NFkB1, DKFZp686C01211, DNA Binding Factor KBF1, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kB, Nuclear Factor kappa B DNA Binding Subunit) (FITC) 100ul　N2303-10-FITC

特徴

• The NFkB family of induced-activated transcription factors are dimers comprised of members of the NFkB /rel protein family.
• Activation of NFkB is induced by a remarkably large number of stimuli including viruses, TNF, IL-1, PMA , LPS, UV light, and others.
• In most cell types, NFkB is present in the cytoplasm as a 50kD (p50) subunit and a 65kD (p65, rel A) subunit.
• In unstimulated cells, nuclear localization signals present on p65 are masked by members of the IkB family of inhibitory proteins.
• Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosomedependant pathway, allowing nuclear translocation of the active dimeric NFkB transcription factor.
• In the nucleus, NFkB binds to consensus sequences where, along with other co-factors, it participates in activating or enhancing the expression of specific genes.
• Applications:Suitable for use in FLISA, Western Blot, Gel Mobility Shift Assay, Immunofluorescence/Immunocytochemistry andI mmunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: paraffin sectionsOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.