Cadherins are a multifunctional family of Ca2+ -dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms.
The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous (1).
Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion (2) .
Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of identical cadherin molecules on neighboring cells.
Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper (3) .
In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins b-catenin and g-catenin which both bind to the actin binding protein a-catenin (4,5) .
The interaction of b-catenin with the cytoplasmic tail of cadherins and other cytoplasmic proteins, including Tcf-family transcription factors and the tumor suppressor protein APC, is thought to be mediated through a region of the b-catenin molecule containing multiple repeats of the 42 amino acid armadillo sequence motif (6).
In addition to playing important roles in differentiation and tissue morphogenesis, cadherins also appear to play a significant role in modulating tumor invasion and metastasis (7).
The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells.
In addition, the tissue specificity of cadherin subtypes are becoming valuable markers for the identification and differential diagnosis of certain cancers (8,9).
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
For Formalin fixed, paraffin embedded sections, Heat Induced Epitope Retrieval (HIER) step is required prior to staining
Western Blot: 1ug/ml ELISA: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：chicken
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes all members of the cadherin family of proteins including N-cadherin, E-cadherin, P-cadherin and R-cadherin. C0107-10A is useful as both a ubiquitous cadherin probe, as well as a marker for adherens junctions. Species crossreactivity: human, bovine, mouse, rat, chicken, amphibian (Xenopus), canine, monkey, as well as other species.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Ca Ch Hu Mk Mo Rt Xe
Immunogen：Synthetic peptide derived from the C-terminus of the chicken N-cadherin protein.
