The ZO-1 and ZO-2 proteins share significant sequence homology to each other (51% identity), as well as to several other proteins which include the lethal(1)discs-large(dlg) tumor supressor gene product (dlg-A) of Drosophila, erythrocyte membrane-associated p55 protein, and PSSD-95/SAP90.
These proteins share several conserved domains and are collectively referred to as the MAGUK family.
The regions of homology among this family of proteins include a domain homologous to guanylate kinase (GUK), a single SH3 domain, and a variable number of N-terminal repeated domains termed PDZ domains.
The ZO-1 and ZO-2 proteins are distinct from the other family member in that they possess C-terminal acidic and proline rich regions, and alternatively spliced domains at their C-termini.
Although, ZO-1 and ZO-2 are structurally similar and display significant sequence homology, the proteins are divergent at their C-termini.
This suggests that the C-terminal ends of ZO-1 and ZO-2 have distinct functions.
The canine ZO-2 protein has a predicted molecular mass of ~132kD.
However, the protein migrates with a mass of 160kD when analyzed by SDS-PAGE.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.1-1ug/ml Western Blot: 1-4ug/mlImmunohistochemistry (Mouse liver tissues): 4-5ug/mlNote: Weak fixation of tissues with 2% paraformaldehyde/picric acid followed by NO post-fixation is required to produce staining
Optimal dilutions to be determined by the researcher.
Positive Control:HeLa and canine MDCK cell lysates and mouse liver homogenates.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Specific for the canine ZO-2 protein (~160kD). Does exhibit crossreactivity with a few proteins of unknown identity in the 50-85kD region. Does not appear to crossreact with the related ZO-1 protein. Species crossreactivity: human and mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo
Immunogen：Synthetic peptide derived from the central portion of the ZO-2 protein.
