84-3652-79　［取扱停止］Vascular Endothelial Growth Inhibitor (VEGI) (FITC) 100ul　V2121-35-FITC

特徴

• Vascular endothelial growth inhibitor (VEGI) belongs to the human tumor necrosis factor (TNF) family and acts as a multifunctional cytokine.
• VEGI is expressed predominantly in endothelial cells and is an endothelial cell-specific negative regulator of angiogenesis.
• Human VEGI protein contains 174 amino acids.
• The calculated molecular weight is approximately 20kD.
• An experiment examining in vitro transcription and translation involving the use of a cDNA clone as a template revealed a similar molecular weight of approximately 22kD.
• Hydrophobicity analysis predicts a 13 amino acid hydrophobic region that follows the C-terminal segment of 12 residues; this is typical of a type II transmembrane protein with the C-terminus on the extracellular surface, a single transmembrane domain, and a short cytoplasmic tail.
• Northern blots reveal that VEGI transcripts have been observed in the endothelial cells of placenta, lung, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine, and colon.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.1-1.0ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.