IL-8, also known as CXCL8, is produced by a range of cells including stimulated monocytes, mast cells, endothelial cells, keratinocytes and fibroblasts.
Human IL-8 promotes neutrophil chemotaxis and degranulation.
Applications:Suitable for use in ELISA, Western Blot, Neutralization Studies and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-2ug/ml; Use at 100ul/well, detects 0.2-0.4ng human IL-8/well.
Western Blot: 0.1-0.2ug/ml detects 1.5-3.0ng human IL-8per lane under reducing and non-reducing conditions.
Neutralization Studies: 2-3ug/ml is found to yield half-maximal inhibition of the biological activity of 100ng/ml human IL-8
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Recognizes all forms of natural and recombinant human IL-8.
Isotype：IgG
Calc Applications Abbrev：E IHC N WB
Calc Crossreactivity：Hu
Immunogen：Highly purified (>98% pure) recombinant protein corresponding to 17aa of human endothelial IL-8.
