Signaling events leading to transcriptional activation initiated by members of the TGF-beta superfamily are known to be mediated by SMAD proteins.
Biological activities mediated by SMADs include cell growth and morphogenesis, development, and immune response.
Activation by TGF-b is mediated by Smad2 and Smad3 while activation by bone morphogenetic protein (BMP) is mediated by Smad 1 and Smad 5.
In contrast to the activating effects of these Smad proteins, Smad6 and Smad7, whose expression is also induced by ligand binding, appear to function as negative regulators of TGF-b superfamily signaling pathways.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: 0.1-1ug/mlWestern Blot: 1-3ug/mlImmunohistochemistry: 1ug/ml Requires HIER with EDTA, pH 8.0 prior to staining
Optimal dilutions to be determined by the researcher.
Positive Controls:Western Blot: human Smad3-transfected COSImmunohistochemistry: human normal breast, esophagus, skin, and breast cancer tissues.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by epitope affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Recognizes human Smad3. The antibody shows very minor cross-reactivity with Smad2 (using relatively high concentrations of recombinant Smad2).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：A 20 amino acid synthetic peptide derived from a central portion of the linker domain of human Smad3.
