Of the 11 families of phosphodiesterase (PDEs) identified, PDE4, PDE7, and PDE8 are specific for cAMP.
However, isoforms of the PDE4 family often account for most of the cAMP-hydrolyzing activity of a cell.
Four genes (PDE4A, B, C and D) make up the PDE4 family.
One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells.
In the CNS, PDE4B subtype variants are expressed in varying ratios.
Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants.
Applications:Suitable for use in Immunoprecipitation and Western Blot.
Other applications have not been tested.
Recommended Dilution;Immunoprecipitation: 7ug/mlWestern Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Reacts with the human and rat PDE4B protein. On Western blots, it identifies a single band at ~83kD. Bands of unknown origin may be observed at ~160 and ~260kD. Reactivities have been confirmed with NG96 human neuroblastoma lysate and rat brain homogenate.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide derived from the N-terminal region of human and rat PDE4B protein.
