84-3652-43　Occludin (Biotin) 100ul　O5204-Biotin

特徴

• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.1-1ug/mlWestern Blot: 1-3ug/mlImmunofluorescence: 4-20ug/mlImmunoprecipitation: ~5ug/IP reactionImmunohistochemistry (FFPE): 2-5ug/ml; Protease pretreatment of sections is required prior to staining.
• Dilute protease to 1-2mg/ml and incubate at 37°C for 10 minutes.
• Other enzyme and HIER pretreatment methods may not be suitable for this antibody
• Optimal dilutions to be determined by the researcher.
• Recommended IF fixation and staining protocol:1.“Pre-extract” the cells on ice for 2 min with an ice-cold buffer containing 0.2% Triton X-100
• 2.
• Fix cells in 3% paraformaldehyde in PBS for 30 min at RT
• 3.
• Permeabilize sample with 0.05% Triton X-100 in PBS for 5 min on ice
• 4.
• Incubate samples with the desired concentration of O5204 (4-20ug/ml) on ice for 1 hour
• 5.
• Incubate with IgG (FITC) Pab Gt x Rb for 30 min at RT.
• Recommended Secondary Antibodies:I1904-40D: IgG, H&L (FITC) Pab Gt xRbI1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb I1904-46N: IgG, H&L (FITC) Pab Gt xRb
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.