84-3652-32　Glucose Regulated Protein 94 (Grp94, gp96) (Biotin) 100ul　G3057-43-Biotin

特徴

• The tumor rejection antigen (gp96), also known as glucose-regulated protein 94 (GRP94), is a member of the heat shock protein (HSP) family.
• The gp96 protein is up-regulated by stress conditions such as glucose starvation and heat shock, and its function is regulated through a ligand-dependent conversion from an active to an inactive conformation.
• gp96 is involved in both innate and adaptive immunity.
• A study has shown that a B-cell line deficient in gp96 is unresponsive to microbial stimuli because toll-like receptors (TLRs) are retained intracellularly.
• However, normal surface levels of TLRs were restored when these cells were transfected with a cDNA encoding gp96.
• The tumor rejection antigen can activate the release of nitric oxide by antigen presenting cells (APCs) and it also has a relevant role in the regulation of immune responses to pathological cell death.
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilution:ELISA: 0.1-1ug/mlWestern Blot: 1-3ug/ml.
• Identifies a single band at ~96kDImmunoprecipitation: 1-5ug/mlImmunohistochemistry: 0.5-1ug/ml Heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining for formalin-fixed, paraffin-embedded (FFPE) tissues
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.