Claudin-18 is a tight junction protein expressed as lung- and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene.1 The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor.1 A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human.1 Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein.1 Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells.1 These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach.1 Claudin-18 expression has also been reported in the inner ear.2
Applications:Suitable for use in Western Blot, Immunofluorescence, and Immunohistochemistry Other applications not tested.
Recommended Dilution:Immunohistochemistry: frozen and paraffin sectionsOptimal dilutions to be determined by the researcher.
Positive Control: Mouse lung and stomach homogenates
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Recognizes mouse Claudin-18 protein at ~29 kD. Species crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide derived from the C-terminal region of the human Claudin-18 protein, which differs from mouse by 4 conservative amino acid replacements and one non-conservative amino acid replacement
