Tight junctions are intercellular junctions that act as both the barrier (or gate), and the fence.
The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces.
The fence function maintains cell polarity, thus, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane.
Tight junctions are comprised of the membrane proteins occludin, claudins, and JAMs.
Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton mediated by ZO-1.
Claudin 12 expression has been observed in the lung, brain, kidney, skin, urinary bladder and ear.
Applications:Suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Positive Control:Mouse lung, colon, and kidney homogenates.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Recognizes mouse Claudin 12. Species Crossreactivity: human and rat
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12
