The c-kit proto-oncogene is the cellular homologue of the feline sarcoma v-kit oncogene.
It encodes a type III transmembrane receptor tyrosine kinase also known as CD117 or stem cell factor receptor (SCFR).
c-Kit signaling is activated when a ligand known as steel factor or stem cell factor (SCF) binds to the receptor.
Interactions between specific phosphotyrosine residues of activated receptor tyrosine kinases (RTKs) and Src homology 2 (SH2) domains define cellulargrowth and differentiation signals.
It has been reported that SCF stimulates the association of the p85 subunit of PI3K with the phosphorylated tyrosine-721 residue within wild type kit.
Subsequently, PI3K contributes to the activation of Akt and JNKs.
c-Kit is necessary for the development of hematopoietic progenitors, mast cells, germ cells, melanocytes, and the interstitial cells of Cajal (ICC).
Oncogenic c-kit mutations have been implicated in neoplasms arising from these stem cell lineages, such as gastrointestinal stromal tumor (GIST).
The mature, phosphorylated form of c-Kit is a glycosylated protein with a molecular weight of ~145kD, while the immature form is ~125kD.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry: 5-10ug/mlNote: Prior to immunohistochemistry with paraffin-embedded tissue sections, heat induced epitope retrieval with EDTA, pH 8.0
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by epitope affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Reacts with the human c-Kit (CD117 or stem cell factor receptor (SCFR)) oncoprotein. On Western blots, this antibody identifies a ~145kD band representing the mature (phosphorylated, glycosylated) form of c-Kit, a ~125kD band for the immature form, and a ~95kD band representing the soluble form. Reactivity has been confirmed with human GIST-882 and EWS-502 cell lysates.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from an internal sequence of the human c-Kit protein
