Autophagy is the major pathway for proteins and organelles being delivered to the lysosome or vacuole for degradation.
Autophagy-mediated degradation may be important during mammalian embryogenesis, differentiation, aging, and non-apoptotic programmed cell death.
Dysfunction in autophagic degradation has been observed in a number of genetic diseases, particularly in cancer, and may also be associated with neurodegenerative conditions such as Parkinson’s and Alzheimer’s disease.
The Apg12 system is a ubiquitin-like protein conjugation system, first discovered in autophagy-defective yeast mutants, that is conserved in eukaryotes.
Covalent attachment of Apg12-Apg5 is required for autophagy; the Apg7 and Apg10 enzymes that catalyze this attachment are analogous to the E1 and E2 enzymes of the ubiquitin-proteasome degradation pathway.
Applications:Suitable for use in ELISA, Immunoprecipitation and Western Blot.
Other applications have not been tested.
Recommended Dilutions:ELISA: 0.1-1ug/mlImmunoprecipitation (Native): 7ug/reactionWestern Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Recognizes human Apg12 protein at ~22kD, as well as additional bands between 40 and 80kD of unknown origin. Reactivity has been confirmed with Apg12-transfected, overexpressed HEK293 cell lysates. Species Sequence Homology: mouse.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the internal region of the human Apg12 protein.
