Ubiquitin carboxyl-terminal hydrolase L1 (UCH–L1), also known as PGP 9.5, is a neuronal and neuroendocrine-specific protein that plays a role in proteosomal protein degradation, which is critical for neuronal health.
UCH-L1 is also involved in p27kip1 degradation through its interaction and nuclear translocation with JAB1.
UCH-L1 expression is specifically associated with non small-cell lung cancer development and it is found in serum of patients with lung cancer, indicating that it may be important in lung cancer screening and diagnosis UCH-L1 may also be a useful marker for invasive colorectal cancer.
Mutations in the alpha-synuclein and Uch-L1 genes cause autosomal dominant Parkinson’s disease (PD).
Derangements in the ubiquitin proteasomal pathway may play important roles in the demise of dopamine neurons in PD.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.1-1ug/mlImmunohistochemistry (Paraffin): 1-2ug/ml; For staining with formalin-fixed paraffin-embedded tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining
Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Recognizes the ~26kD human UCH-L1 protein. A faint band of unknown origin may also be observed at ~50kD. Reactivity has been confirmed with mouse and rat brain homogenates, NGP neuroblastoma lysates, A549 lung carcinoma lysates, and NCI-H69 lung carcinoma lysates. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from the C-terminal region of human UCH-L1. Species Sequence Homology: mouse, rat, equine; 100%
