Human aurora A (aurora related kinase-1 (ARK-1), Aik1, STK15, BTAK, aurora2), and human aurora B (aurora related kinase-2 (ARK-2), Aik2, STK12, aurora1) are centrosome-associated serine/threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis.1, 2 Aurora B has unique genetic loci relative to Aurora A suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression.1, 3 Aurora B exists in a complex with survivin and inner centromere protein (INCENP) in mitosis, and its kinase activity is stimulated by survivin binding and cell cycle-dependent phosphorylation.4 Phosphorylation of INCENP by aurora B stimulates aurora B kinase activity .5
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1-3ug/mWestern Blotting: 1-3ug/mlImmunoprecipitation: 7ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Epitope-affinity purified from rabbit antiserum.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Reacts with the human Aurora B. On Western blots, it identifies the target band at ~39kD. A band of unknown origin is also observed at ~ 75kD. Reactivity has been confirmed with human HeLa cervical carcinoma cell lysates.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the N-terminal region of the human Aurora B protein.
