ZO-3, a ~130kD protein, contains three PSD-95/SAP90, discs-large, ZO-1 (PDZ) domains, a SH3 domain, and a guanylate kinase-liked domain.
Like ZO-1 and ZO-2, ZO-3 contains a C-terminal acidic domain and a basic region (between the first and second PDZ domains), making it belongs to the MAGUK protein family.
By Western Blot analysis, ZO-3 is identified as a ~130kD protein that directly interacts with tight junction proteins ZO-1 and occuldin, but not with ZO-2.
However, in one study, ZO-2, ZO-3 and Occludin colocalized with actin aggregates at cell border in MDCK cells.
ZO-3 is localized to sites of cell-cell interaction, identical to that of ZO-1 in tight junctions.
By Immunoprecipitation analysis, ZO-3 co-immunoprecipitates with ZO-1.
Using MDCK cells, ZO-3 protein colocalized with ZO-1 by Immunofluorescence.
In vitro binding assays, the N-terminal of ZO-3 showed preferably binding of ZO-1 and actin filaments.
Moreover, the binding of ZO-1/ZO-2/ZO-3 was also found to interact with certain Claudin proteins.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 0.5-2ug/ml ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Immunoprecipitation: 2-3ugOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Recognizes human ZO-3 protein with a molecular weight of ~130kD. Crossreactivity with other Zonula Occluden family members has not been observed. Reactivity is confirmed with human T84, HeLa, and canine MDCK cell lysates.
Isotype：IgG
Calc Applications Abbrev：E IF IP WB
Calc Crossreactivity：Ca Hu
Immunogen：Synthetic peptide derived from the internal region of the human ZO-3 protein.
