Estrogen receptor (ER) is a member of the steroid-receptor family.
Unlike protein growth factors that bind to receptors on the cell surface and activate signal-transduction cascades to influence gene expression, the steroid hormones bind to intracellular receptors, which then bind to DNA and regulate gene expression directly.
ER-b has a molecular mass ofapproximately 53kD and is composed of six regions, A to F, on the basis of different amino acid sequence homology (2).
The A/B region contains a transcription activation function.
Region C encodes the DNA binding domain.
Region D is involved in binding to hsp90, as well as containing nuclear localization signals and plays a part in stabilizing DNA binding by the DNA binding domain.
Region E contains the hormone/ligand binding domain as well as hormone-inducible transcription activation function.
Region F appears to play a role in modulating transcriptional activation by ER-a.
ER-b was discovered in 1996 in rat prostate, as well as in secretory epithelial cells of the prostate and granulose cells of the ovary (1).
Later, human ER-b was discovered and characterized and found to be expressed in human thymus, spleen, ovary and testis (3).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Recognizes human Estrogen Receptor beta protein (~53kD). Reactivity was confirmed by Western Blot using recombinant human Estrogen Receptor beta and MCF-7 cell lysate.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：A 19 amino acid synthetic peptide derived from the C-terminus of the human Estrogen Receptor beta protein.
