Mitogen activated protein kinases (MAPKs) play pivotal roles in mediating signal transduction from the cell surface to the nucleus (1) .
These kinases are encoded by distinct genes and together form a family of kinases whose activation is dependent upon dual phosphorylation on specific threonine and tyrosine residues.
In yeast, a number of different MAP Kinases have been identified and are activated by distinct signaling pathways
Applications:Suitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Reacts strongly with ERK1 and ERK2. A synthetic peptide, corresponding to a 15 amino acid sequence near the carboxyl terminus (339–353 aa of rat ERK1) was conjugated to carrier protein and used as the immunogen. Reactivity to ERK1 and ERK2 has been confirmed by western blot analysis of human A431 and K562 cells, mouse brain and rat brain. Human, mouse and rat (positive control: human A431 and K562 cells, mouse brain and rat brain cell lysates).
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide (a.a. 339–353) corresponding to a C-terminal segment of rat ERK1.
