c-MET (HGFr) is a proto-oncogene (locus 7q21-q31) protein and a cell-surface receptor of hepatocyte growth factor.
It has tyrosine kinase activity.
It consists of alpha (50kD) and beta(145kD) subunits linked by a disulfide bond.
c-Met is overexpressed in many cancers.
The MET gene is often amplified between tumorgenesis and metastasis.
It has been suggested that c-Met is useful as a prognostic marker in numerous cancers including: breast cancer, gastric carcinoma, endometrial carcinoma and others.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded sections.
Epitope retrieval required using citrate buffer, pH 6.0 is required.
Enzyme digestion is not required
Optimal dilutions to be determined by the researcher.
Positive Control:Breast carcinoma
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Recognizes human c-Met. Detects a 45kD protein on Western Blots of A431 cell lysate.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the human c-Met C-terminus sequence, conjugated to a carrier protein. Expected Staining Pattern: Cytoplasmic
