Following its characterization as an activated oncogene (based on its ability to transform normal fibroblasts) Met was characterized as the receptor for hepatocyte growth factor.
The c-Met receptor is a disulfide bonded heterodimer consisting of an ~50kD extracellular a-chain and an ~145kD b-chain with a cytoplasmic tyrosine kinase domain.
Both chains are derived from a single precursor protein by proteolytic cleavage.
c-Met is expressed in a wide variety of adult and embryonic tissues.
It transmits biological responses including mitogenesis, morphogenesis and anti-apoptotic activity elicited by HGF.
Dysregulated c-Met gene expression is observed in a variety of human carcinomas and sarcomas.
c-Met also mediates the movement and invasiveness of neoplastic cells and promotes metastasis.
Recently, transcription of the c-Met gene was suggested to be a target for regulation by p53.
Applications:Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: paraffin sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Specificity：Recognizes the human c-Met protein at ~145kD. Does not crossreact with related proteins.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa located within last 50aa derived from the C-terminus of the human c-Met protein.
