84-3651-64　Cullin 2 (CUL-2) (Biotin) 100ul　C7949-13-Biotin

特徴

• In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by a ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1 and one of many F-box proteins (reviewed in ref 2).
• A family of human cullin genes homologous to the S.
• cerevisiae cdc53 gene has been identified (cul1, 2, 3, 4a, 4b, and 5) (1) .
• It was then shown that Cul1 forms a complex with human Skp1 and the F box protein Skp2.
• In contrast, Cul2 does not associate with Skp1 and Skp2.
• Instead it forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongin C, Elongin B and VHL (2) .
• The meaning of this association is still unknown.
• Intriguingly, Elongin C shows homology with Skp1 and Elongin B with ubiquitin.
• In addition, in its active form, SIII contains, instead of VHL, an F box protein: Elongin A.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:ELISA (Immunogen): 0.1-1.0ug/ml Western Blot: 1-3ug/ml Immunoprecipitation (Native): 5-10ug Optimal dilutions to be determined by the researcher.
• Positive Control:CUL2 recombinant protein and cell lysates
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.