Tight junctions are intercellular junctions that act as both the barrier (or gate), and the fence.
The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces.
The fence function maintains cell polarity, thus, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane.
Tight junctions are comprised of the membrane proteins occludin, claudins, and JAMs.
Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton mediated by ZO-1.
Claudin 12 expression has been observed in the lung, brain, kidney, skin, urinary bladder and ear.
Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Positive Control:Mouse lung, colon, and kidney homogenates.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes mouse Claudin 12. Species Crossreactivity: human and rat
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12
