Cdc14, also known as duel specificity phosphatase, is a protein phosphatase originally discovered in yeast that plays an important role in cell cycle regulation, including exit from mitosis (1).
Throughout G1, S and G2 phases, Cdc14 is located in the nucleolus; at the beginning of anaphase, the protein is redistributed throughout the entire nucleus (2).
In late anaphase, Cdc14 dephosphorylates Cdh1, which then activates APC (Anaphase Promoting Complex) (3).
Two human orthologs of yeast Cdc14 have been identified; these are Cdc14A and Cdc14B, and they share about 80% amino acid homology in the N-termini and phosphatase domains (4).
Cdc14 has also been shown to dephosphorylate p53, suggesting that it may play a role in regulation of p53 activity.
Applications:Suitable for use in Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilution:Immunoprecipitation: 1-3ug/mlWestern Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Positive Controls:Human HeLa cell lysates
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes the human Cdc14B protein including isoforms 1, 2 and 3. The calculated molecular weight of Cdc14B isoforms range from 53-60kD. The apparent molecular weight of Cdc14B in HeLa cells was ~66kD. Reactivity is confirmed with human HeLa cell lysates.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the N-terminal region of the human Cdc14B protein
