The c-kit proto-oncogene is the cellular homologue of the feline sarcoma v-kit oncogene.
It encodes a type III transmembrane receptor tyrosine kinase also known as CD117 or stem cell factor receptor (SCFR).
c-Kit signaling is activated when a ligand known as steel factor or stem cell factor (SCF) binds to the receptor.
Interactions between specific phosphotyrosine residues of activated receptor tyrosine kinases (RTKs) and Src homology 2 (SH2) domains define cellulargrowth and differentiation signals.
It has been reported that SCF stimulates the association of the p85 subunit of PI3K with the phosphorylated tyrosine-721 residue within wild type kit.
Subsequently, PI3K contributes to the activation of Akt and JNKs.
c-Kit is necessary for the development of hematopoietic progenitors, mast cells, germ cells, melanocytes, and the interstitial cells of Cajal (ICC).
Oncogenic c-kit mutations have been implicated in neoplasms arising from these stem cell lineages, such as gastrointestinal stromal tumor (GIST).
The mature, phosphorylated form of c-Kit is a glycosylated protein with a molecular weight of ~145kD, while the immature form is ~125kD.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlImmunohistochemistry: 5-10ug/mlNote: Prior to immunohistochemistry with paraffin-embedded tissue sections, heat induced epitope retrieval with EDTA, pH 8.0
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by epitope affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Reacts with the human c-Kit (CD117 or stem cell factor receptor (SCFR)) oncoprotein. On Western blots, this antibody identifies a ~145kD band representing the mature (phosphorylated, glycosylated) form of c-Kit, a ~125kD band for the immature form, and a ~95kD band representing the soluble form. Reactivity has been confirmed with human GIST-882 and EWS-502 cell lysates.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from an internal sequence of the human c-Kit protein
