Autophagy is the major pathway for proteins and organelles being delivered to the lysosome or vacuole for degradation.
Autophagy-mediated degradation may be important during mammalian embryogenesis, differentiation, aging, and non-apoptotic programmed cell death.
Dysfunction in autophagic degradation has been observed in a number of genetic diseases, particularly in cancer, and may also be associated with neurodegenerative conditions such as Parkinson’s and Alzheimer’s disease.
The Apg12 system is a ubiquitin-like protein conjugation system, first discovered in autophagy-defective yeast mutants, that is conserved in eukaryotes.
Covalent attachment of Apg12-Apg5 is required for autophagy; the Apg7 and Apg10 enzymes that catalyze this attachment are analogous to the E1 and E2 enzymes of the ubiquitin-proteasome degradation pathway.
Applications:Suitable for use in ELISA, Immunoprecipitation and Western Blot.
Other applications have not been tested.
Recommended Dilutions:ELISA: 0.1-1ug/mlImmunoprecipitation (Native): 7ug/reactionWestern Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human Apg12 protein at ~22kD, as well as additional bands between 40 and 80kD of unknown origin. Reactivity has been confirmed with Apg12-transfected, overexpressed HEK293 cell lysates. Species Sequence Homology: mouse.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the internal region of the human Apg12 protein.
