Wnts have diverse roles in governing cell fate, proliferation, migration, and polarity during development, particularly in embryogenesis and carcinogenesis in adults1-3.
Wnts are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity (also called ‘planar polarity’)2, 4,5 pathways.
Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of these pathways and lead to unique cellular responses.
The Wnt/b-catenin pathway primarily regulates cell fate determination during development, whereas the main function of the Wnt polarity pathway is the regulation of cytoskeletal organization.
Wnt2B/Wnt13 is expressed in the embryonic mesoderm during gastrulation, and in the dorsal midline of the diencephalons and mesencephalon, the heart primordial, the lung bud periphery, and in the otic and optic vesicles at later stages.6 Wnt2B is expressed in organs regulated by epithelial-mesenchymal interactions in the mouse embryo, such as the developing kidney, lung, salivary gland, gut, pancreas, adrenal gland, genital tubercle, eye and ear.7 Wnt2B signaling induces ureter branching during early kidney organogenesis.7 Wnt2B exists as 2 alternatively spliced isoforms (Wnt2B1 and Wnt2B2).8 Wnt2B2 is preferentially expressed in NT2 cells, with the potential of neuronal differentiation.9 In some cases of gastric cancer, Wnt2B2 up-regulation may lead to carcinogenesis through the activation of the b-catenin/TCF signaling pathway.9 Wnt2B is expressed in the immature ovary, where the Wnt signaling cascade may be involved in follicular development and ovarian function.10
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blotting: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Epitope-affinity purified from rabbit antiserum.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Reacts with the human, mouse and rat Wnt2B/Wnt13 proteins. It recognizes both human Wnt13A and Wnt13B isoforms. On Western blots, it identifies a band at ~41kD. Reactivity has been confirmed with mouse Wnt2B/Wnt13-pCMV-tag2-transfected 293T, human Wnt13A- and Wnt13B-pCR3-transfected HEK293 and SK-OV-3 cell lysates, and rat ovary homogenates.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from an internal region of the human Wnt2B/Wnt13 protein.
