84-3650-63　［取扱停止］Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) (APC) 100ul　U1005-01D-APC

特徴

• Ubiquitin carboxyl-terminal hydrolase L1 (UCH–L1), also known as PGP 9.5, is a neuronal and neuroendocrine-specific protein that plays a role in proteosomal protein degradation, which is critical for neuronal health.
• UCH-L1 is also involved in p27kip1 degradation through its interaction and nuclear translocation with JAB1.
• UCH-L1 expression is specifically associated with non small-cell lung cancer development and it is found in serum of patients with lung cancer, indicating that it may be important in lung cancer screening and diagnosis UCH-L1 may also be a useful marker for invasive colorectal cancer.
• Mutations in the alpha-synuclein and Uch-L1 genes cause autosomal dominant Parkinson’s disease (PD).
• Derangements in the ubiquitin proteasomal pathway may play important roles in the demise of dopamine neurons in PD.
• Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.1-1ug/mlImmunohistochemistry (Paraffin): 1-2ug/ml; For staining with formalin-fixed paraffin-embedded tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining
• Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: APC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.