The Ras/Raf/MEK/ERK signaling pathway conveys mitogenic and differentiation signals from the cell membrane to the nucleus, and controls the proliferation and differentiation of different cell types.1–3 The Raf kinase inhibitor protein (RKIP) interacts with Raf1, MEK, and ERK both in vitro and in vivo,4 interfering with this signaling cascade.
RKIP’s mechanism of action has been identified; it inhibits the Raf/MEK/ERK pathway at the Raf-1/MEK interface, where it dissociates the Raf-1/MEK complex and acts as a competitive inhibitor of MEK phosphorylation by Raf.5
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1–1.0ug/mlWestern Blot: 1–3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Reacts with human and mouse RKIP. In Western Blots, identifies a single 23kD band. Reactivity has been confirmed with human HEK293 cell lysates and mouse brain homogenates. Based on amino acid sequence homology, expected to react with bovine and monkey RKIP.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Hu Mk Mo
Immunogen：Synthetic peptide derived from the N-terminal region of human RKIP (Raf kinase inhibitor protein).
