Intercellular communication through gap junctions (connexins) plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation and growth control.
In rodents, 15 connexin genes have been described.
Specific cell types in the brain express specific types of connexins; expression patterns coincide with tissue compartmentalization and function.
These compartments change during development5.
Reduction or alteration in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis.
The coding sequence of the mouse gap junction Cx47 has 49% homology with mouse Cx45; both are located on chromosome 11 in the mouse genome.
Cx47 has been observed to express mainly in the gray matter of the central nervous system, specifically in several types of hippocampal, cortical, cerebellar and spinal cord neurons.
Functional gap junctions are involved in tissue homeostasis of ions, metabolites and second messenger molecules.
Between neuronal cells, these functions play a role in synchronizing oscillations of certain cell clusters and could impact burst thresholds of electrotonically coupled cells.
Cx47 induces strong electrical coupling that is sensitive to chemical uncouplers.
Cx47 appears to be a less restrictive channel than Cx36 and may show preference to larger metabolites or second messengers rather than the intercellular diffusion of inorganic ions that Cx36 preferentially allows.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-3ug/mlImmunohistochemistry (Frozen): 2-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes the mouse Connexin 47 protein. On Western Blots of mouse brain homogenates, it identified a single band at ~47kD. Reactivity has been confirmed in Western Blots with mouse thalamus, spinal cord, hypothalamus, cerebellum and whole brain lysates.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to the C-terminal region of the mouse Connexin 47 protein.
