Intercellular communication mediated by gap junctions plays an important role in a variety of cellular processes, including homeostasis, morphogenesis, cell differentiation, and regulation of growth.1–3 Gap junctions are transmembrane channels that directly link neighboring cells by regulating the exchange of low molecular weight (<1200D) metabolites, ions, and second messengers.
Gap junctions are formed by the interaction of hemichannels (connexons) on adjacent cells.
Each connexon is composed of a hexameric assembly of connexin proteins.
Connexins are highly homologous proteins encoded by a multigene family with similar structural features, including a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops, and a C-terminal cytoplasmic tail.
Comparison of the amino acid sequence of the various connexin family members indicates that the two areas of greatest divergence are the intracellular loop connecting the second and third transmembrane domains and the C-terminal tail.1–2 These distinct domains are thought to mediate connexin type-specific properties, including phosphorylation, responses to gating stimuli, and assembly and membrane turnover.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes mouse connexin 32 in liver and brain. On Western Blot with mouse liver cell lysates, Cx32 exhibits a band at 32kD. Reactivity is confirmed with mouse liver lysates. Species sequence homology: human and rat Cx32.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide derived from the C-terminal sequence of the mouse connexin 32 protein.
