Alterations and deletions within the FHIT (Fragile Histidine Triad) gene and concomitant perturbations of FHIT gene expression are strongly linked to the genesis and establishment of human tumors of the lung (1–3), cervix (4,5), breast (6,7), stomach (8), pancreas (9), and other tissues.
Originally identified by collaborating researchers in the U.S.
and Japan (10), the > 500 kbp FHIT gene was shown to span the fragile chromosomal site FRA3b (11) at band 3p14.2.
These researchers and subsequent others demonstrated that loss of heterozygousity or homozygous deletion at this locus are characteristics of multiple tumor-derived cell lines including those from colon, stomach, and breast.
Further evidence that FHIT acts as a tumor suppressor in normal cells comes from experiments showing that tumorigenesis of cancer cells is inhibited by replacement of the FHIT gene (12).
The involvement of other genes within the FRA3B region in tumor suppression has also been suggested (6).
In a comparative study of lung cancers from heavy smokers and non-smokers, alterations in the FHIT gene were found at much higher rates (80% vs.
22%) in tumors from heavy smokers (2).
Thus, FHIT may be a ready target for carcinogens present in cigarette smoke.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.1–1.0ug/ml Western Blot: 0.3–1.0ug/ml Immunohistochemistry: 0.5–3.0ug/ml; Formalin-fixed, paraffin embedded sections require a Heat Induced Epitope Retrieval step (HIER)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human FHIT at ~17kD. Reactivity has been confirmed by Western Blot using lysates from rat brain, colon, spleen, stomach, kidney, lung, small intestine and testes as well as 293T cells and COS cells transfected with a pCMV-FHIT-FLAG construct. Species Crossreactivity: Rat. Does not react with mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Full length human FHIT fusion protein with GST (glutathione amino transferase)
