84-3650-39　Estrogen Receptor, beta (ERb) (APC) 100ul　E3565-17-APC

特徴

• Estrogen receptor (ER) is a member of the steroid-receptor family.
• Unlike protein growth factors that bind to receptors on the cell surface and activate signal-transduction cascades to influence gene expression, the steroid hormones bind to intracellular receptors, which then bind to DNA and regulate gene expression directly.
• ER-b has a molecular mass ofapproximately 53kD and is composed of six regions, A to F, on the basis of different amino acid sequence homology (2).
• The A/B region contains a transcription activation function.
• Region C encodes the DNA binding domain.
• Region D is involved in binding to hsp90, as well as containing nuclear localization signals and plays a part in stabilizing DNA binding by the DNA binding domain.
• Region E contains the hormone/ligand binding domain as well as hormone-inducible transcription activation function.
• Region F appears to play a role in modulating transcriptional activation by ER-a.
• ER-b was discovered in 1996 in rat prostate, as well as in secretory epithelial cells of the prostate and granulose cells of the ovary (1).
• Later, human ER-b was discovered and characterized and found to be expressed in human thymus, spleen, ovary and testis (3).
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: APC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.