84-3650-33　Lysosome Associated Membrane Protein 2 (LAMP 2) (APC) 100ul　L9190-12-APC

特徴

• Lamp-2 (lysosomal associated protein 2) has been demonstrated to be involved in the direct uptake of cytosolic proteins in lysosomes and also participates in the endocytic pathway.
• The protein is localized mainly to lysosomes.
• Under certain conditions, it can also be detected in the plasma membrane.
• Interestingly, the level of Lamp-2 protein expressed on the surface of tumor cells has been found to correlate with the tumor’s metastatic potential.
• In lysosomes, Lamp-2 is localized to the lysosomal lumen and is inserted in the membrane via a single 20 amino acid transmembrane domain.
• The caboxy-terminal portion of the protein (12 amino acids) is located on the cytosolic side of the lysosomal membrane.
• The Lamp-2 protein is ubiquitously distributed and evolutionarily conserved.
• Only minor changes in the transmembrane and cytosolic domains have been detected among various species.
• Note: the rat protein has been called lgp96, while the human form of the protein has been called Lamp-2.
• Applications:Suitable for use in ELISA and Western Blot.
• Able to completely block transport of cytosolic proteins into lysosomes in rat derived cells.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-1ug/mlWestern Blot: 0.1-1.0ug/ml e.g.
• 90ng/ml for 100ug rat liver lysosomal protein
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: APC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.