Sprouty-1 and -2 are membrane-anchored phosphoprotein inhibitors of growth factor signaling in endothelial cells.
Four mammalian Sprouty homologues (Sprouty-1–4) have been identified.
One study showed that overexpressed Sprouty-1 and -2 inhibits fibroblast growth factor– and vascular endothelial growth factor–induced proliferation and differentiation by repressingpathways leading to MAP kinase activation.1 Sprouty proteins are also endogenous inhibitors of the Ras/MAP kinase pathway that play an important role in the remodeling of branching tissues, such as in development of the lung, kidney tubules, vascular system, and breast ducts
A recent study shows that Sprouty-1 and –2 may have tumor suppressing activity in the breast, and found that both proteins are consistently down-regulated in breast carcinomas.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilution to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Alkaline Phosphatase (AP).
Specificity：Recognizes mouse Sprouty-1 at ~34kD. A band of unknown origin was observed at ~120kD. Reactivity has been confirmed with two day-old mouse kidney homogenates. Does not recognize rat Sprouty-1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide derived from the N-terminal region of mouse and rat Sprouty-1
