In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by a ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1 and one of many F-box proteins (reviewed in ref 2).
A family of human cullin genes homologous to the S.
cerevisiae cdc53 gene has been identified (cul1, 2, 3, 4a, 4b, and 5) (1) .
It was then shown that Cul1 forms a complex with human Skp1 and the F box protein Skp2.
In contrast, Cul2 does not associate with Skp1 and Skp2.
Instead it forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongin C, Elongin B and VHL (2) .
The meaning of this association is still unknown.
Intriguingly, Elongin C shows homology with Skp1 and Elongin B with ubiquitin.
In addition, in its active form, SIII contains, instead of VHL, an F box protein: Elongin A.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:ELISA (Immunogen): 0.1-1.0ug/ml Western Blot: 1-3ug/ml Immunoprecipitation (Native): 5-10ug Optimal dilutions to be determined by the researcher.
Positive Control:CUL2 recombinant protein and cell lysates
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Alkaline Phosphatase (AP).
Specificity：Recognizes human CUL2 protein at ~83kD. Does not crossreact with the related CUL1 protein.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the C-terminus of the human CUL2.
