In yeast, proteolysis of G1 cell cycle regulators is controlled by a ubiquitin ligase formed by three subunits: Cdc53, Skp1 and one of many F-box proteins (1).
Human Skp1 was first identified as a protein associated with Cyclin A (2).
The meaning of Skp1-Cyclin A association is still understood.
It was then shown that it forms a complex with human Cdc53 (Cul1) and the F box protein Skp2.
It is possible that this complex plays a role in the ubiquitination of G1 regulatory proteins as its homolog does in yeast.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunodepletion.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-1.0ug/ml Western Blot: 1-2ug/mlImmunohistochemistry: 2-5ug/mlImmunoprecipitation: 5ug/IP reaction (immunodepletion reactions may require more antibody) Optimal dilutions to be determined by the researcher.
Positive Control:HeLa, Jurkat and 293T cells.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Alkaline Phosphatase (AP).
Specificity：Recognizes human p45SKP2. Does not crossreact with related endogenous proteins. Based on sequence homology, reactivity of this antibody in species other than human is likely.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant, full-length human p45SKP2 protein.
