The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development.
Pax family members possess a DNA-binding domain encoded by the paired box.
Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program.
The paired box containing genes were first identified in Drosophila and were subsequently described in a variety of species including mouse, human, chicken, Xenopus and Zebrafish.
Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6 is associated with Aniridia, and pax-2 which is associated with Wilms tumor.
During embryogenesis, Pax-2 is expressed in the developing kidney.
In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures.
Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms’ tumors.
Persistent expression of Pax-2 in Wilms’ tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors.
Interestingly, expression of the Wilms’ tumor suppresser protein WT1 coincides with down-regulation of the pax-2 gene, and the WT1 protein can directly repress pax-2 transcription.
Therefore, pax-2 is thought to be a target gene for WT1.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Alkaline Phosphatase (AP).
Specificity：Recognizes mouse Pax2. Does not crossreact with other Pax family members. The major epitopes are located between aa204-373 of mouse Pax2. Two proteins are detected in mouse embryonic kidney lysates: a major band at 46kD (Pax2b) and a minor band at 48kD (Pax2a). Species Crossreactivity: ELISA: Immunogen; WB: Xenopus, zebrafish, embryonic mouse kidney, human Wilms’ tumors; IHC (frozen): rat, Xenopus, zebrafish, human Wilms’ tumor, mouse; IHC (paraffin): human Wilms’ tumor, mouse; IP: Embryonic mouse kidney; sheep and zebrafish.
Isotype：IgG
Calc Applications Abbrev：E IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt Sh Xe Ze
Immunogen：A GST-Pax-2 fusion protein corresponding to aa188-385 from the C-terminal of mouse Pax2.
